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, Ming Lin School of Pharmaceutical Sciences, Zhejiang Chinese Medical University , Binwen Road, Binjiang District, Hangzhou 310053 , People’s Republic of China Zhejiang Conba Pharmaceutical Co., Ltd , Binkang Road, Binjiang District, Hangzhou 310051 , People’s Republic of China Search for other works by this author on: Oxford Academic Chun-ling Xu Zhejiang Conba Pharmaceutical Co., Ltd , Binkang Road, Binjiang District, Hangzhou 310051 , People’s Republic of China Search for other works by this author on: Oxford Academic Hong-ye Pan Zhejiang Conba Pharmaceutical Co., Ltd , Binkang Road, Binjiang District, Hangzhou 310051 , People’s Republic of China Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology , Binkang Road, Binjiang District, Hangzhou 310051 , People’s Republic of China College of Pharmaceutical Sciences, Zhejiang University , Yuhangtang Road, Xihu District, Hangzhou 310058 , People’s Republic of China Search for other works by this author on: Oxford Academic Yong-biao Song Inner Mongolia Conba Pharmaceutical Co., Ltd , Sini Town, Hangjin Banner, Erdos City, Inner Mongolia Autonomous Region, 017418 , People’s Republic of China Search for other works by this author on: Oxford Academic Yi-wen Ma Inner Mongolia Conba Pharmaceutical Co., Ltd , Sini Town, Hangjin Banner, Erdos City, Inner Mongolia Autonomous Region, 017418 , People’s Republic of China Search for other works by this author on: Oxford Academic Xing-yu Liu Zhejiang Conba Pharmaceutical Co., Ltd , Binkang Road, Binjiang District, Hangzhou 310051 , People’s Republic of China Search for other works by this author on: Oxford Academic Jian-biao Yao Zhejiang Conba Pharmaceutical Co., Ltd , Binkang Road, Binjiang District, Hangzhou 310051 , People’s Republic of China Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology , Binkang Road, Binjiang District, Hangzhou 310051 , People’s Republic of China Author to whom correspondences should be addressed. Email: yaojb@conbapharm.com and wangruwei1967@163.com Search for other works by this author on: Oxford Academic Ru-wei Wang School of Pharmaceutical Sciences, Zhejiang Chinese Medical University , Binwen Road, Binjiang District, Hangzhou 310053 , People’s Republic of China Author to whom correspondences should be addressed. Email: yaojb@conbapharm.com and wangruwei1967@163.com Search for other works by this author on: Oxford Academic
Journal of Chromatographic Science, Volume 62, Issue 8, September 2024, Pages 732–741, https://doi.org/10.1093/chromsci/bmae015
Published:
28 March 2024
Article history
Received:
31 January 2023
Revision received:
10 November 2023
Accepted:
14 March 2024
Published:
28 March 2024
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Ming Lin, Chun-ling Xu, Hong-ye Pan, Yong-biao Song, Yi-wen Ma, Xing-yu Liu, Jian-biao Yao, Ru-wei Wang, Quality Evaluation of Shexiang Tongxin Dropping Pill Based on HPLC Fingerprints Combined with HPLC-Q-TOF-MS/MS Method, Journal of Chromatographic Science, Volume 62, Issue 8, September 2024, Pages 732–741, https://doi.org/10.1093/chromsci/bmae015
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Abstract
Shexiang Tongxin Dropping Pill (STP) is a composite formula of traditional Chinese medicine that is widely used for the treatment of cardiovascular diseases. It consists of seven medicinal extracts thereof or materials, including Bufonis venenum, synthetic Moschus, Panax ginseng, Bovis calculus artifactus, Bear bile powder, Salvia miltiorrhiza Bge and synthetic borneol. However, it is considerably difficult to evaluate the quality of STP due to its complex chemical compositions. This paper was designed to explore a comprehensive and systematic method combining fingerprints and chemical identification for quality assessment of STP samples. Twenty batches of STP samples were analyzed by high-performance liquid chromatography (HPLC) and high-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry. Ten common peaks were detected by HPLC fingerprint similarity evaluation system. Meanwhile, 100 compounds belonging to 4 structural characteristics, including 23 bufadienolides, 36 organic acids, 34 saponins and 7 other types, were systematically identified as the basic components in STP. This study could be used for clarifying the multiple bioactive substances and developing a comprehensive quality evaluation method of STP.
© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com
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