Quality Evaluation of Shexiang Tongxin Dropping Pill Based on HPLC Fingerprints Combined with HPLC-Q-TOF-MS/MS Method (2024)

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Volume 62 Issue 8 September 2024
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Ming Lin

School of Pharmaceutical Sciences, Zhejiang Chinese Medical University

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Binwen Road, Binjiang District, Hangzhou 310053

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People’s Republic of China

Zhejiang Conba Pharmaceutical Co., Ltd

,

Binkang Road, Binjiang District, Hangzhou 310051

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People’s Republic of China

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Chun-ling Xu

Zhejiang Conba Pharmaceutical Co., Ltd

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Binkang Road, Binjiang District, Hangzhou 310051

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People’s Republic of China

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Hong-ye Pan

Zhejiang Conba Pharmaceutical Co., Ltd

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Binkang Road, Binjiang District, Hangzhou 310051

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People’s Republic of China

Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology

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Binkang Road, Binjiang District, Hangzhou 310051

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People’s Republic of China

College of Pharmaceutical Sciences, Zhejiang University

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Yuhangtang Road, Xihu District, Hangzhou 310058

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People’s Republic of China

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Yong-biao Song

Inner Mongolia Conba Pharmaceutical Co., Ltd

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Sini Town, Hangjin Banner, Erdos City, Inner Mongolia Autonomous Region, 017418

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People’s Republic of China

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Yi-wen Ma

Inner Mongolia Conba Pharmaceutical Co., Ltd

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Sini Town, Hangjin Banner, Erdos City, Inner Mongolia Autonomous Region, 017418

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People’s Republic of China

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Xing-yu Liu

Zhejiang Conba Pharmaceutical Co., Ltd

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Binkang Road, Binjiang District, Hangzhou 310051

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People’s Republic of China

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Jian-biao Yao

Zhejiang Conba Pharmaceutical Co., Ltd

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Binkang Road, Binjiang District, Hangzhou 310051

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People’s Republic of China

Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology

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Binkang Road, Binjiang District, Hangzhou 310051

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People’s Republic of China

Author to whom correspondences should be addressed. Email: yaojb@conbapharm.com and wangruwei1967@163.com

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Ru-wei Wang

School of Pharmaceutical Sciences, Zhejiang Chinese Medical University

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Binwen Road, Binjiang District, Hangzhou 310053

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People’s Republic of China

Author to whom correspondences should be addressed. Email: yaojb@conbapharm.com and wangruwei1967@163.com

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Journal of Chromatographic Science, Volume 62, Issue 8, September 2024, Pages 732–741, https://doi.org/10.1093/chromsci/bmae015

Published:

28 March 2024

Article history

Received:

31 January 2023

Revision received:

10 November 2023

Accepted:

14 March 2024

Published:

28 March 2024

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    Ming Lin, Chun-ling Xu, Hong-ye Pan, Yong-biao Song, Yi-wen Ma, Xing-yu Liu, Jian-biao Yao, Ru-wei Wang, Quality Evaluation of Shexiang Tongxin Dropping Pill Based on HPLC Fingerprints Combined with HPLC-Q-TOF-MS/MS Method, Journal of Chromatographic Science, Volume 62, Issue 8, September 2024, Pages 732–741, https://doi.org/10.1093/chromsci/bmae015

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Abstract

Shexiang Tongxin Dropping Pill (STP) is a composite formula of traditional Chinese medicine that is widely used for the treatment of cardiovascular diseases. It consists of seven medicinal extracts thereof or materials, including Bufonis venenum, synthetic Moschus, Panax ginseng, Bovis calculus artifactus, Bear bile powder, Salvia miltiorrhiza Bge and synthetic borneol. However, it is considerably difficult to evaluate the quality of STP due to its complex chemical compositions. This paper was designed to explore a comprehensive and systematic method combining fingerprints and chemical identification for quality assessment of STP samples. Twenty batches of STP samples were analyzed by high-performance liquid chromatography (HPLC) and high-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry. Ten common peaks were detected by HPLC fingerprint similarity evaluation system. Meanwhile, 100 compounds belonging to 4 structural characteristics, including 23 bufadienolides, 36 organic acids, 34 saponins and 7 other types, were systematically identified as the basic components in STP. This study could be used for clarifying the multiple bioactive substances and developing a comprehensive quality evaluation method of STP.

© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/pages/standard-publication-reuse-rights)

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